Characterization of Regenerative Phenotype of Unrestricted Somatic Stem Cells (USSC) from Human Umbilical Cord Blood (hUCB) by Functional Secretome Analysis
نویسندگان
چکیده
منابع مشابه
Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres
BACKGROUND Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigat...
متن کاملDifferentiation of Somatic Unrestricted Cord Blood Stem Cells into Chondrocyte Cells
Purpose: To isolate and purify unrestricted somatic stem cells from human umbilical cord blood and evaluation of their differentiation into chondrocyte in vitro.Materials and Methods: In this study cells from human umbilical cord blood were isolated and plated in flask. Colonies were performed after one week. To determine the kind of cells, 100000 cells were analysed with flowcytometry. Twenty ...
متن کامل3d culture of umblical cord blood-drived cd34+ cells on a dbm scaffold coated by cord blood–derived unrestricted somatic stem cells (ussc)
objective: allogeneic transplantation with umbilical cord blood (ucb) in adult recipients is limited mainly by a low cd34+ cell dose. to overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (mpc)- unrestricted somatic stem cells (ussc)- was incorporated in an attempt to expand cd34+ cells from ucb. to provide a similar environment in vitro, we coated ...
متن کاملMigratory capabilities of human umbilical cord blood-derived neural stem cells (HUCB-NSC) in vitro.
Many types of neural progenitors from various sources have been evaluated for therapy of CNS disorders. Prerequisite for success in cell therapy is the ability for transplanted cells to reach appropriate target such as stroke lesion. We have established neural stem cell line from human umbilical cord blood neural stem (HUCB-NSC). In the present study we evaluated migratory capabilities of cells...
متن کاملNanofiber Expansion of Umbilical Cord Blood Hematopoietic Stem Cells
Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2015
ISSN: 1535-9476
DOI: 10.1074/mcp.m115.049312